Le séquençage de l’ADNr 16S situe les isolats dans 18 genres bactériens. Les plages moyennes d’inhibition de la croissance du mycélium varient de 1 à 12 mm et le degré moyen d’inhibition de la germination des zoospores, de 0 à 100 pour cent. La sélection in vitro de bactéries telluriques a débouché sur l’identification de 184 isolats antagonistes, dont 22 d’une collection de cultures existante. euteiches, et établir si on pourrait s’en servir comme moyen de lutte biologique contre le piétin du pois de grande culture en chambre de croissance. Les auteurs voulaient isoler puis identifier les bactéries du sol susceptibles d’agir in vitro contre le mycélium et les zoospores d’ A. Ce champignon nuit parfois de façon importante à la production de cette légumineuse dans l’Ouest canadien. RésuméĪphanomyces euteiches est un agent pathogène du sol responsable du piétin du pois. This study demonstrates the possibility of aphanomyces root rot management using biocontrol agents. Further screening of 20 isolates as soil inoculants identified K-Hf-L9 ( Pseudomonas fluorescens), PSV1-7 ( Pantoea agglomerans), and K-Hf-H2 ( Lysobacter capsici) isolates as having the highest biocontrol activity, significantly ( α = 0.05) suppressing aphanomyces root rot in field pea in growth chamber trials. that significantly ( α = 0.05) suppressed aphanomyces root rot in field pea grown in vermiculite, suggesting the intriguing possibility of using N-fixing Rhizobium inoculants as biocontrol agents for aphanomyces control. Screening of 47 bacteria that inhibited both infective stages identified 29 potential biocontrol strains, including Rhizobium spp. Use of 16S rDNA sequence analysis placed isolates into 18 different bacterial genera. Mean mycelial growth inhibition zones ranged from 1 to 12 mm, and mean zoospore germination inhibition ranged from 0% to 100%.
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In vitro screening of soil bacteria identified 184 antagonistic isolates, including 22 from an existing culture collection. euteiches mycelial and zoospore developmental stages under in vitro conditions and assess their potential as biocontrol agents against aphanomyces root rot in field pea under growth chamber conditions. This study aimed to isolate and identify soil bacteria with antagonistic activity towards A. Aphanomyces euteiches is a soil-borne pathogen that causes root rot of pea and can significantly affect pea production in western Canada.